TSK-GEL® 簡介

Extensively used in laboratories all over the world, our TSK-GEL® is designed for researchers seeking the highest level of performance. Covering the total range of HPLC, this product is made from small, spherical particles and offers high resolution (even at high flow rates), excellent reproducibility, and long column life. Scaling up from analytical to preparative columns is made simple and easier.

 

TSK-GEL® Column分類

Separation mode

TOSOH column Type

Applications

GFC 

SW type

PW type

Proteins, enzymes, saccharides, nucleic acids, DNA, water soluble synthetic polymers 

IEC 

IEC-5PW

IEC-2SW

IEC-3SW

IEC-NPR series 

Proteins, enzymes, nucleic acids, peptides, DNA 

HIC 

HIC-5PW series butyl-NPR 

Proteins, enzymes 

RPC 

Silica-base series

PW series 

Octadecyl-NPR 

Low MW organic compounds, peptides, proteins, oligonucleotides 

AFC 

AFC-5PW series 

Proteins, nucleic acids 

ERC 

Enantio series 

α-Amino acids, amines 

GPC 

H type 

Organic compounds, synthetic polymers, oligomers 

IC 

IC type 

Cations, anions 

 

TSK-GEL® SW GFC Column

Calibration curves of 

the TSKgel SW GFC columns

Features of TSKgel SW type columns



 

◎ Hydrophilic surface with hydroxyl group 

◎ Low adsorptivity to proteins and enzymes. 

◎ Column series with appropriate pore size for separating proteins and enzymes 

◎ Improved mechanical strength 

◎ Applicable to various solvents 

◎ Stable in eluent of pH from 2 to 7 

◎ XL series column shows remarkably high performance, e.g. sharp peak, higher resolution, due to narrow distribution of pore and pore size and particle size. 

◎ Glass columns are available for purification. 

◎ Three ranges:1x105、5x105、7x106 (proteins) 

◎ Main applications: Proteins、enzymes、nucleic acids

 

TSK-GEL® PW GFC Column

Calibration curves of the PW type packed columns

Features of TSKgel PW type columns:

◎ It has a hydrophilic surface with hydroxyl group.

◎ It has very little absorption in water-soluble synthetic polymer and polysaccharides.

◎ It has very wide fractionation range of molecular weights.

◎ It is highly stable and durable.

◎ It has excellent chemical stability and can be used over a wide pH range, between 2 and 12.

◎ The XL series, which is particularly stable and durable, can measure even with 50% organic solvent.

◎ Main applications:

(1) Water-soluble synthetic oligomers, Polyethylene glycol, etc.

(2) Water-soluble synthetic polymer, Polyvinyl alcohol, polyacryl amide, etc.

(3) Oligosaccharides and poly- Saccharides, dextran, dextrin and starch

(4) Nucleic acid: DNA, RNA and plasmid

PW type packed columns' list:

Description 

MWt Exclusion Limit (Dextran)

TSKgel G2500PW(XL) 

5x103

TSKgel G3000PW(XL) 

2x105 

TSKgel G4000PW(XL)

1x106

TSKgel G5000PW(XL)

2.5x106

TSKgel G6000PW(XL)

5x107 (est.) 

TSKgel GMPW(XL) 

5x107 (est.) 

TSKgel G-Oligo-PW

5x103*

TSKgel G-DNA-PW

Below 7000 base paris

● Polyethylene glycol (PEG)

● Packing solvent:H2O

● Guard columns & Reference columns are not listed here, please consult us.

 

TSK-GEL® H GPC Column

Calibration curves of the
TSKgel HXL GPC columns

Features of TSKgel HXL columns:

◎ These columns cover wide fractionation range of sample from low to high molecule weight.

◎ It allows little swelling and shrink, thus ensuring a long column life.

◎ It allows for excellent stablilty and reproducibility.

◎ The HXL series have high number of theoretical plates and high resolution due to narrow distribution of particle size.

◎ Main applications: Polymers soluble in organic solvents、Oligomers、General organic compounds and polymer additives.

◎ Supply mode of solvents: Solvents can be shipped as chloroform, dimethylformamide (DMF) and acetone, as well as tetrahydrofuran(THF). Please specify when ordering. (Note) Carefully read the instructions manual attached to the supplied column when you replace the solvent.

H type packed columns’ list:

Description

MWt Exclusion Limit (polystyrene)

TSKgel G1000H(XL),H6,H8

1x103

TSKgel G2000H(XL),H6,H8XL

1x104

TSKgel G2500H(XL),H6,H8

2x104

TSKgel G3000H(XL),H6,H8

6x104

TSKgel G4000H(XL),H6,H8

4x105

TSKgel G5000H(XL),H6

4x106

TSKgel G6000H(XL),H6

4x107(est.)

TSKgel G7000H(XL),H6

4x108(est.)

TSKgel GMH(XL),H6

4x108(est.)

TSKgel GMH(XL)-HT, GMH6-HT

(High Temp)

4x108(est.)

TSKgel GMHXL-L

4x108(est.)

●  Guard columns & Reference columns are not listed here, please consult us.

●  The differences between H, HXL, H6, H8 are column ID, length & particle size.

※ TSKgel HR columns for organic solvent GPC has been developed with yhe further improvement on higher stability against various solvent exchange. 

※ TSKgel Super H columns has improved further more on the performance of HR columns. It could provide the same performance by half elution volume of HR columns and could save solvent consumption to a third. 

※ HM-N series: mixed-bed columns.

 

 

 

 

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