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Thermo Fisher Co. HPLC Columns :

　

　

　

Thermo Fisher 分離管選擇指南

Before starting an analysis, consider the physical and chemical properties of the analyte(s) and how the analyte(s) will react with the surface of the chromatographic phase. To aid column selection, the following guide may be useful：

Molecular Weight	Solubility		Mode	Column
<2000	Organic	Hexane	Adsorption	Hypersil 120 Silica Hypersil 100 Silica
		Hexane +Alcohol	Polar phase	Hypersil APS-2 Hypersil CPS Hypersil BDS Cyano Hypersil Phenyl Hypersil BDS Phenyl HyPURITY Cyano
		Alcohol	Reversed-phase	Hypersil SAS Hypersil MOS Hypersil ODS Hypersil 100 C8 Hypersil 100 C18 Hypersil BDS C8 Hypersil BDS C18 Hypersil Phenyl Hypersil BDS Phenyl HyPURITY C18 HyPURITY Advance HyPURITY C8
	Water Soluble	Non-Ionic	Polar phase	Hypersil CPS Hypersil Phenyl HyPURITY Cyano Hypersil BDS Cyano Hypersil BDS Phenyl
			Reversed-phase	Hypersil SAS Hypersil MOS Hypersil ODS Hypersil Phenyl Hypersil 100 C8 Hypersil 100 C18 Hypersil BDS C8 Hypersil BDS C18 Hypersil BDS Phenyl HyPURITY C18 HyPURITY Advance HyPURITY C8
			Wide-pore reversed-phase	Hypersil PEP 300 C18 HyPURITY C4
		Ionic	Ion exchange	Hypersil Duet C18/SAX Hypersil SAX Hypersil APS-2
>2000	Water Soluble	Ionic	Ion exchange	HyperREZ XP SAX HyperREZ XP SCX Vydac 300VHP Vydac 301VHP Vydac 400VHP
		Non-Ionic	Wide-pore reversed-phase	Hypersil PEP 300 C4 Hypersil PEP 300 C18 HyPURITY C4

　

生化分析用Thermo Fisher 分離管選擇指南

	MW	Mode	Column
Proteins		Wide-pore reverse phase	Hypersil PEP 300 C4, HyPURITY™ C4, Hypersil PEP 300 C18
Proteins		Ion-exchange	Hypersil WP 300 SAX
Peptides	<2000	Reverse phase	Hypersil PEP 100 C8, Hypersil PEP 100 C18, HyPURITY C4, C8, C18
	>2000	Wide-pore reverse phase	Hypersil PEP C4, Hypersil PEP 300 C18
		Ion-exchange	Hypersil WP 300 SAX
		Ion-exchange	Hypersil WP 300 SAX
Amino acids (derivatised)		Reverse phase	Hypersil Amino Acid
Oligonucleotides		Wide-pore reverse phase	Hypersil PEP 300 C3, Hypersil PEP 300 C18, Hypersil PEP
Nucleotides		Reverse phase	Hypersil ODS, HyPURITY™ C4
		Ion-exchange	Hypersil SAX, Hypersil WP 300 SAX
		Ion-exchange	Hypersil SAX, Hypersil WP 300 SAX
Nucleotides		Reverse phase	Hypersil ODS, Hypersil C18, Hypersil BDS C18

　

　

　

　

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