Thermo Fisher Co. HPLC Columns :

　

         

　

~ Thermo Fisher 各系列分析管柱  簡介 ~

　

　

 

　

　

■   HyPURITY & HyPURITY ADVANCE

　

•HyPURITY提供最完整的確效報告：

 1. 充填材物性檢測：含碳量、作用表面積、平均粒徑、孔積…等

 2. 分析效能檢測

 3. 批次穩定度證明

 4. 品質控制測試：檢測項目如下表

        Table: VALIDATION SUMMARY

Test

Characteristic Diagnosed Method

Steric Selectivity

 Phase selectivity Relative retention of polyaromatic hydrocarbons
Hydrophobicity Hydrophobic character and surface coverage of bonded phase Retention and selectivity of non-polar hydrocarbons
Hydrogen Bonding Capacity Measure of residual silanol groups at silica surface Selectivity factor of caffeine relative to phenol
Ion Exchange Capacity at pH 2.7 Peak tailing caused by ion exchange  and sites Retention of benzylamine
Ion Exchange Capacity at pH 7.6 Peak tailing caused by ion exchange  and dissociated silanol sites Retention of benzylamine
Analysis of Bases at pH 7 Effect of dissociated silanols on basic  analytes peak symmetry and retention Analysis of tricyclic antidepressants,
Analysis of Chelators Peak tailing caused by by active metal/silanol sites Chromatography of acids and chelators

<<最詳盡而完整的確效報告，符合藥廠需求！>>

 

HyPURITY擁有別人所沒有的特長：

* ISO9001嚴格控管，採用純度達99.999%的Silica，重金屬含量 

     最低！

* 分析效能極佳！波峰對稱性極佳！

 

* 適用廣泛的pH值àpH 0.9~9   ★

* 應用範圍廣泛：              ★

   1. 酸性、中性、鹼性樣品皆可分析且波峰對稱性佳！

   2. 金屬螯合化合物分析

   3. 藥物分析ex. Antidepressants、Antihistamines、 

       Antibacterials

   4. Peptides、Vitamins分析

* 最優良的管柱穩定性，管柱使用壽命極長！

* 5 phases－C18、C8、C4、Cyano、AQUASTAR

Phase

Particle Size(μm)

Surface Area (m2/g)

Pore Size (Å)

% Carbon

C18

3,5

200

180

13

C8

5

200

180

8

C4

5

200

180

4.5

Cyano

5

200

180

4

* 可用於LC/MS

  

　

■    Hypercarb

• Enhanced selectivity for closely related compounds such as geometric isomers, diastereoisomers, polar solutes and PCB’s

• Physical properties similar to a wide pore spherical silica but with 100% Carbon

• Excellent for the separation of highly polar compounds

• Unique Porous Graphitic Carbon Material --- the selectivity offered is different to that of silica and polymeric phases

• Stability at extreme conditions of pH (10M acid to 10M alkali), salt concentration and temperature

• Excellent batch-to-batch reproducibility

• Homogeneous surface

• 3D Shape Selective

• 極性差距大之樣品可一次分離

 

　

★★★請點選 此處 獲得Hypercarb更多的產品訊息!★★★

　

 

　

　

■ Hypersil GOLD    您必買的頂級Column

 

Specification  Phase

C %

Pore Size (Ã)

Surface Area (m2/g)

Based on Pure Silica

Hypersil GOLD

10%

175

220

99.99%

 

理由一：  Unrivaled Peak Shape                                           理由二：  Critical Resolution

 

 

 

 

 

 

 

 

 

 

 

 

　

　

　

　

　

　

             Hypersil GOLD columns offer improved peak                                  Resolution of analytes is improved using a

                  shape even for basic analytes                                                        Hypersil GOLD column

　

　

理由三：  pH Stability                                                               理由四：  Enhanced Sensitivity

　

                                                                         

　

　

　

　

　

　

　

　

　

　

　

　

　

　

　

　

　

　

　

　

　

　

　

　

　

                 High pH stability(pH 10.6) of Hypersil GOLD columns                              Hypersil Gold produces optimum

                                                                                                             peak shapes for analytes, therefore

                                                      providing maximum peak height for

                                                                                                              sensitivity of trace compounds.                                                                             

                                        

特性：                                                                                    

◎ 有絕佳的峰型。                             ◎ 滯留效果佳，無拖尾的現象。    

◎ 高選擇性和高解析度。                     ◎ 採用99.99%高純度Silica 。

◎ 敏感度高，效率佳。                        ◎ No Bleed。

◎ 極佳的pH穩定性。

　

■   Hypersil BDS 系列

• Base deactivated --- reduces the effect of the polar silanol groups

• Reduced tailing

• Reduces the need for mobile phase additions

• Excellent peak symmetry

• Long column lifetimes

• All phases are end-capped

• Excellent performance with basic, neutral and acidic compounds

• C18、C8、Phenyl、Cyano phases are available

 

 

　

■    Hypersil Classics 系列

• Reliable and reproducible columns for polar and neutral compounds

• All columns supplied with test certification

• Precise column efficiency specification

• Long column lifetime

• Wide range of bonded phases：silica, ODS, ODS-2, MOS(C8) & MOS-2, phenyl & phenyl-2, SAS(C1),  APS-2, CPS & CPS-2, SAX

• One of the worlds most widely referenced column packing materials with a proven track record. They are extremely popular materials having in excess of 1500 literature references.

 

 

　

■    Hypersil 100 (HS) 系列

• High retention

• Large surface area for long retention of polar compounds

• Ideal for scale up to prep or process applications with the same chemistry using Hyperprep HS column

• High carbon loading gives a robust, stable phase

• Base deactivated surface

• Suitable for acidic, basic and neutral compounds

• Wide range of bonded phases：silica, C18, C8, phenyl, APS, CPS and provide 5um particle size

 

 

　

■    Hypersil GREEN 系列

• Hypersil GREEN ENV --- for environmental analysis, specially tailored for polar pollutants 

• Hypersil GREEN ENV --- excellent selectivity for phenols, phthalates, herbicides

• Hypersil GREEN PAH --- dedicated to the analysis of Polynuclear Aromatic Hydrocarbons (PAHs)

• Hypersil GREEN PAH --- complete resolution of EPA 610 mix in less than 4 minutes

• Hypersil GREEN CARBAMATE --- broad spectrum carbamate resolution

• Hypersil GREEN CARBAMATE --- Ideal packing for EPA method 531.1

 

 

　

■    Hyperbond 

• 材質同 Waters uBondapak

 

 

　

■    Hypersil DUET 系列

• Mixed mode column and 2 phases available：C18/SCX & C18/SAX

• Ideal choice for discovery compounds and drug metabolism

• For separation of hydrophobic and ionic compounds in a single run

• DUET column eliminate the need for column switching, ion-pairing or ion suppression, or for using column in series

• Retention of ionisable compounds adjusted by pH

 

 

　

■    Hypersil PEP 系列

• Columns for peptide and protein analysis and isolation

• Scale up with Hyperprep PEP column for preparative and process work

• 100Å and 300Å pore sizes available，phases：C18, C8, C4

 

 

　

■    Hypersil AMINO ACID 

• Operates with a wide range of derivatisation profiles

• Excellent resolution of amino acids

• The Hypersil AMINO ACID column is specially designed to separate a range of amino acids of areas including clinical chemistry, the food industry and the pharmaceutical industry

 

 

　

■    HyperREZ 系列

• HyperREZ XP CARBOHYDRATE column

   --- Typical applications：Carbohydrates, saccharides, alcohols, organic acids

   --- Stable at low pH

   --- Simple column clean-up

Phase

Counter Ion

Particle size (μm)

% Cross linking

HyperREZ XP Carbohydrate

H+, Pb++, Ca++

8

8

HyperREZ XP Carbohydrate

Na+, Ag+

10

4

• HyperREZ XP RP Reverse phase column

   --- Typical applications：polar compounds, trace enrichment, synthetic peptides, small  

        proteins

   --- Rigid Polystyrene/divinylbenzene spherical particles ~ strong retentive packing

   --- Easily sterilized

   --- Columns available with 100and 300Å media、5μm

• HyperREZ XP Ion Exchange column

   --- Typical applications：peptides, enzyme proteins, basic proteins, protein fingerprinting, recombination protein purification, mono/polyclonal antibody separation, fingerprinting protein profiles, molecular biology, DNA/RNA, small molecules、nucleotides、peptides

   --- The HyperREZ XP SCX is optimized for macromolecule separations using a sulphonic acid derivatisation

   --- pH range 1-13 and high physical and chemical stability

   --- 1000 Å， SCX & SAX phases

　

　

 

　

Thermo Electron尚備有：

微量型分析管柱

製備型分析管柱

毛細分離管柱

CEC分離管柱

GPC分析管柱

Chiral分析管柱

LC/MS分析管柱

SPE分離管柱

  可供選擇！

　

　

　

 

　

　

　

　

　

<   接   下   頁   >

　

         

　

<<回高效能液相層析儀(HPLC)>>   <<回HPLC分析管柱>>