|
As
columns for sugar analyses SHODEX, Asahipak NH2P-50, GS-220 HQ,
RSpak DC-813, USPpak MN-431 and SUGAR series can be used. In
addition to these, normal phase columns, such as Silica 5NH, are
also suitable.
|
Series name
|
Applications
|
|
SUGAR
S series
|
Effective
analyses are possible for sugars and sugar alcohols using
mixed modes such as size exclusion, ion exchange, ligand
exchange and partition & adsorption.
|
|
SUGAR
KS series
|
Suited
for analyses of sugars by size exclusion. Since grades of a
wide pore size range are available, wide applications from
monosaccharides to polysaccharides are possible.
|
|
Asahipak GS-220 HQ
|
Suited
for analyses of oligosaccharides by size exclusion.
|
|
Asahipak
NH2P-50
RSpak
DC-613
|
Suited
for analyses of monosaccharides, disaccharides,
oligosaccharides and sugar alcohols by partition &
adsorption.
|
|
USPpak
MN-431
|
Can
be used for mannitol analysis according to the USP method
(United States Pharmacopeia 23 ).
|
¡@
|
Column type
|
Counter
ion
|
Spearation1
mode
|
Exclusion
limit
(pullualn)
|
Plate
number
|
ID x Length
(mm)
|
|
SUGAR SH1011
SUGAR SH1821
SUGAR
SH-G (SH1011P)
|
H+
H+
H+
|
SEC + IEX
SEC + IEX
(guard column)
|
1,000
10,000
|
>15,000
>15,000
|
8.0 x 300
8.0 x 300
6.0 x
50
|
|
SUGAR SC1011
SUGAR SC1821
SUGAR
SC-LG (SC1011P)
|
Ca2+
Ca2+
Ca2+
|
SEC + LEX
SEC + LEX(guard column)
|
1,000
10,000
|
>12,000
>12,000
|
8.0 x 300
8.0 x 300
6.0 x
50
|
|
SUGAR SP0810
SUGAR SP-G
(SP0810P)
|
Pb2+
Pb2+
|
SEC + LEX
(guard column)
|
1,000
|
>10,000
|
8.0 x 300
6.0 x
50
|
|
SURAR SC1211
SUGAR SC-G
(SC1211P)
|
Ca2+
Ca2+
|
P & A + LEX
(guard column)
|
¡Ð
|
>5,000
|
6.0 x 250
4.6 x
10
|
|
SUGAR SZ5532
SUGAR
SZ-G (SZ5532P)
|
Zn2+
Zn2+
|
P & A + LEX
(guard column)
|
¡Ð
|
>5,000
|
6.0 X 150
4.6 X
10
|
|
SUGAR
KS-801
SUGAR KS-802
SUGAR KS-803
SUGRA KS-804
SUGAR KS-805
SUGAR KS-806
SUGAR KS-G (KS-800P)
|
Na+
Na+
Na+
Na+
Na+
Na+
Na+
|
SEC +
(LEX)
SEC +
(LEX)
SEC
SEC
SEC
SEC
(guard column)
|
1,000
10,000
50,000
400,000
5,000,000
(50,000,000)2
|
>15,000
>15,000
>15,000
>15,000
>8,000
>8,000
|
8.0 x 300
8.0 x 300
8.0 x 300
8.0 x 300
8.0 x 300
8.0 x 300
6.0 x
50
|
|
Asahipak GS-220 HQ
Asahipak GS-2G 7B
|
¡Ð
¡Ð
|
SEC
(guard column)
|
3,000
|
>16,000
|
7.6 x 300
7.6 x
50
|
|
Asahipak NH2P-50 4D
Asahipak NH2P-50 4E
Asahipak NH2P-50G 4A
Asahipak NH2P-50 2D
Asahipak NH2P-50G 2A
Asahipak
NH2P-LF
|
(NH2)
(NH2)
(NH2)
(NH2)
(NH2)
(NH2)
|
P & A
P & A
(guard column)
P & A
(guard column)
(line filter)
|
¡Ð
¡Ð
¡Ð
|
>5,000
>7,500
>3,000
|
4.6
x 150
4.6
x 250
4.6 x
10
2.0
x 150
2.0 x
10
8.0 x
75
|
|
RSpak DC-613
RSpak DC-G
(DC-613P)
|
Na+
Na+
|
P & A
(guard column)
|
¡Ð
|
>5,000
|
6.0 x 150
4.6 x
10
|
|
.USPpak
MN-431
|
Ca2+
|
SEC +
(LEX)
|
¡Ð
|
¡Ð
|
4.0
x 250
|
1
: SEC: Size exclusion IEX :Ion exclusion LEX : Ligand exchange
P & A :Partition & adsorption
2
: Figures in parentheses are estimated value.
¡@
For
custom columns whose column Size
is not listed here, please consult us.
¡@
¡@
¡@
¡@
Elution Volume of Saccharides
Elution
volumes of saccharides for seven different columns,
SUGAR
SP0810, SUGAR SC1011, SUGAR KS-801, SUGAR SZ5532, SUGAR SC1211,
Asahipak NH2P and Asahipak GS-220 are shown in
the table.
|
Substances
|
Elution
Volume (mL)
|
|
SUGAR
|
Asahipak
|
|
SP0810
|
SC1011
|
KS-801
|
SZ5532
|
SC1211
|
NH2P-50
4E
|
GS-220
HQ
|
|
Pb2+
|
Ca2+
|
Na+
|
Zn2+
|
Ca2+
|
|
N-Acetyl-alpha
-D-glucosamine
|
8.86
|
7.75
|
6.68
|
¡Ð
|
4.10
|
6.66
|
|
|
D(+)-Arabinose
|
10.42
|
8.91
|
8.21
|
5.11
|
5.56
|
6.18
|
|
D-Arabitol
|
15.86
|
11.33
|
7.63
|
7.27
|
8.16
|
6.29
|
|
Aspartame
|
¡Ð
|
¡Ð
|
¡Ð
|
¡Ð
|
34.02
|
¡Ð
|
|
2-Deoxy-D-glucose
|
8.83
|
7.58
|
7.15
|
4.34
|
4.02
|
6.02
|
|
Difructose
anhydride III
|
7.07
|
6.30
|
5.81
|
4.30
|
¡¯
|
7.77
|
|
Dulcitol
|
20.18
|
12.76
|
7.40
|
9.46
|
11.28
|
7.45
|
|
meso-Erythritol
|
12.70
|
10.09
|
7.86
|
5.73
|
6.27
|
5.43
|
17.38
|
|
Ethanol
|
11.13
|
11.33
|
10.09
|
¡Ð
|
4.27
|
¡Ð
|
|
|
1-Fructofuranosyl
| |
